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NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated in 1962 at the New York University School of Medicine Department of Pathology. 3T3 refers to the cell transfer and inoculation protocol for the line, and means “3-day transfer, inoculum 3 x 10 5 cells.” Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture ... R.e.a.d Control of proliferation in animal cells. A virus is a small infectious agent that replicates only inside the living cells of an organism.Viruses can infect all types of life forms, from animals and plants to microorganisms, including bacteria and archaea.. 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