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Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date
methods necessary to study genes in yeast.Procedures are included that enable newcomers to set up a yeast laboratory and ...
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Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. Methods in Enzymology buy Protein solubility is affected by ions. At low ion concentrations (<0.5 M), protein solubility increases along with ionic strength.Ions in the solution shield protein molecules from the charge of other protein molecules in what is known as ‘salting-in’ ().At a very high ionic strength, protein solubility decreases as ionic strength increases in the process known as ‘salting-out’.
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shotgun sequencing is a method used for sequencing long DNA strands. It is named by analogy with the rapidly expanding, quasi-random firing pattern of a shotgun.. The chain termination method of DNA sequencing ("Sanger sequencing") can only be used for short DNA strands of 100 to 1000 base pairs.Due to this size limit, longer sequences are subdivided into smaller fragments that ...
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