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sequencing DNA. DNA is replicated in the presence of chemically altered versions of the A, C, G, and T bases. These bases stop the replication process when they are incorporated into the growing strand of DNA, resulting in varying lengths of short DNA ... PacBio
sequencing captures sequence information during the replication process of the target DNA molecule. The template, called a SMRTbell, is a closed, single-stranded circular DNA that is created by ligating hairpin adaptors to both ends of a target double-stranded DNA (dsDNA) molecule .When a sample of SMRTbell is loaded to a chip called a SMRT cell , a SMRTbell diffuses into a
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sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA
sequencing has been performed using the chain termination method developed by Frederick Sanger.This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.
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Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide.This may serve to identify the protein or characterize its post-translational modifications.Typically, partial
sequencing of a protein provides sufficient information (one or more sequence tags) to identify it with reference to databases of protein sequences derived from ...
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